Identification

Title

Concentrations and antibiotic resistance of faecal indicator organisms and human pathogens associated with sewage waste on Scottish beaches, 2021

Abstract

The data includes concentrations of faecal indicator organisms isolated from the plastic waste and natural substrates found on Scottish beaches, as well as the antibiotic resistance of selected strains of these organisms. Sewage-associated plastic waste, and naturally occurring substrates (seaweed and sand), were collected in 2021 from ten beaches along the Firth of Forth estuary (Scotland, UK). The concentrations of faecal indicator organisms (FIOs), E. coli and intestinal enterococci (IE), and potential human pathogens (Vibrio spp.) on the plastic waste and natural substrates were analysed using selective media. Minimum inhibitory concentration (MIC) analysis was used to determine antibiotic resistance in selected strains. Full details about this dataset can be found at https://doi.org/10.5285/9d0a6437-f01e-4cf3-9f83-86d2f4ead869

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/9d0a6437-f01e-4cf3-9f83-86d2f4ead869

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/9d0a6437-f01e-4cf3-9f83-86d2f4ead869.zip

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/9d0a6437-f01e-4cf3-9f83-86d2f4ead869

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

environment

Keywords

Keyword set

keyword value

Habitats and Biotopes

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Geographic location

West bounding longitude

-3.878

East bounding longitude

-2.747

North bounding latitude

56.277

South bounding latitude

55.848

Temporal reference

Temporal extent

Begin position

2021-12-01

End position

2021-12-31

Dataset reference date

date type

publication

effective date

2024-08-09

Frequency of update

Quality and validity

Lineage

Sewage-associated plastic waste (wipes, cotton bud sticks and sanitary products) was collected from ten beaches. All samples were stored at 4 °C and processed within 48 h. Replicate composite samples of wipes, cotton bud sticks and seaweed from each site were pre-enriched in non-selective media Luria-Bertani (LB) broth or Vibrio selective Bile Peptone broth at 37 °C in a shaking incubator for 18 h. Following pre-enrichment, serial dilutions were carried out in phosphate buffered saline (PBS) and dilutions were plated onto selective media. All plates were inverted and incubated. The presence or absence of target bacteria on all sewage-associated plastic waste samples was determined by enumerating colony forming units (CFUs). Minimum inhibitory concentration (MIC): Representative individual colonies of E. coli, IE and Vibrio spp. isolated from seaweed, wet wipe, and cotton bud stick samples, were picked off agar plates, grown further overnight in LB broth, and subjected to MIC analysis to determine antibiotic resistance to amoxicillin, ampicillin, streptomycin, cephalexin, and tetracycline at varying concentrations. Two-fold serial dilutions of each antibiotic were made in LB broth in 96-well plates. Bacterial cultures were adjusted to an optical density at a wavelength of 600nm (OD600nm) of 0.1, and 10 μL added to each well of a 96-well plate. Samples were incubated overnight at 37 °C.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services