Identification

Title

Flow cytometry data derived from samples collected from the Celtic Sea in 2018

Abstract

These data contain information on the abundance and fluorescence of 6 phytoplankton functional groups based on flow cytometry measurements. The data covers 2 surveys on the RV Cefas Endeavour, CEND05_18 and CEND17_18, both in the Celtic Seas.

Resource type

dataset

Resource locator

https://data.cefas.co.uk/view/20363

name: Cefas Data Portal

description: The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: CSV, ESRI Shapefile. The data can also be accessed via the WFS and WMS protocols.

function: download

Unique resource identifier

code

CEFAS20363

codeSpace

https://data.cefas.co.uk

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

Keywords

Keyword set

keyword value

originating controlled vocabulary

title

SeaDataNet P03 parameter discovery vocabulary

reference date

date type

revision

effective date

2011-03-25

Keyword set

keyword value

originating controlled vocabulary

title

SeaDataNet P02 parameter discovery vocabulary

reference date

date type

revision

effective date

2011-03-25

Keyword set

keyword value

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

originating controlled vocabulary

title

SeaVoX Vertical Co-ordinate Coverages

reference date

date type

revision

effective date

2010-05-18

Keyword set

keyword value

originating controlled vocabulary

title

MEDIN metadata record availability

reference date

date type

publication

effective date

2012-01-11

Keyword set

keyword value

originating controlled vocabulary

title

MEDIN metadata record availability

reference date

date type

publication

effective date

2012-01-11

Geographic location

West bounding longitude

-12.00

East bounding longitude

-2.50

North bounding latitude

52.00

South bounding latitude

46.50

Temporal reference

Temporal extent

Begin position

2018-03-15

End position

2018-11-10

Dataset reference date

date type

publication

effective date

2021-03-31

date type

revision

effective date

2024-01-03

date type

creation

effective date

2020-02-26

Frequency of update

asNeeded

Quality and validity

Lineage

A CytoSense fixed beam scanning flow cytometer, fitted with a 488nm blue argon laser was used to measure phytoplankton size and abundance. The flow cytometer was connected to the ferrybox on board the RV Cefas Endeavour with a continuous water intake at approximately 4m depth. Samples were analysed inline for 10 minutes each hour without the addition of a fixative. Data file cleaning (removal of debris) and calibration (using a microsphere of known size, and a microsphere with strong yellow fluorescence; F8852, Life Technologies, USA) was carried out with CytoClus v3 software (CytoBuoy, the Netherlands). Standardised phytoplankton functional types were agreed with partners in the Jerico next project to be picophytoplankton (broken down into synechococcus and eukaryote picophytoplankton), nanophytoplankton (broken down into coccolithophores, cryptophytes, and eukaryote nanophytoplankton), and microphytoplankton. Abundances of phytoplankton functional types (cell l-1) were estimated based on size classes (from forwards scatter measurements of each cell) and particle fluorescence. Locations for samples were obtained from the FerryBox system.

Conformity

Conformity report

specification

title

INSPIRE Data Specification on Species Distribution – Technical Guidelines

reference date

date type

publication

effective date

2013-12-10

degree

false

explanation

See the referenced specification

Conformity report

specification

title

reference date

date type

publication

effective date

2010-12-08

degree

true

explanation

See the referenced specification

Data format

name of format

Unknown

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Responsible organisations

Responsible party

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

originator

Responsible party

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

custodian

Responsible party

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

distributor

Metadata on metadata

Metadata point of contact

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

pointOfContact

Metadata date

2024-01-03T01:01:36

Metadata language

eng