299f89cd-7bbe-4428-9552-c51981c5f883
English
ISO/IEC 8859-1 (also known as Latin 1)
dataset
dataset
Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
LA1 4AP
UK
info@eidc.ac.uk
pointOfContact
2021-06-25T18:38:56
UK GEMINI
2.3
WGS 84
Heterogeneity and environmental stress resistance of wild yeast isolates
2019-10-10
publication
https://catalogue.ceh.ac.uk/id/299f89cd-7bbe-4428-9552-c51981c5f883
10.5285/299f89cd-7bbe-4428-9552-c51981c5f883
doi:
Hewitt, S., Warry, A., Dyer, P.S., Avery, S.V. (2019). Heterogeneity and environmental stress resistance of wild yeast isolates. NERC Environmental Information Data Centre 10.5285/299f89cd-7bbe-4428-9552-c51981c5f883
This dataset contains analytical results for wild yeast isolates from soil samples at and near a disused metal smelting works in the north-east of the UK. The main contaminant from the smelting works was lead (Pb), but Cd was also elevated. The soil near the smelting works showed above-background levels of Pb. Yeasts of similar colony morphology on laboratory agar were isolated and proved to be mostly isolates of Saitozyma podzolica: 56 independent clonal isolates of this yeast were confirmed by ITS sequencing and RAPD-PCR. All of the S. podzolica isolates were tested for Pb and Cd resistance (IC50) and heteroresistance. Heteroresistance indicated variation in metal resistance between individual cells of clonal populations. Some isolates were also tested with Zn.
The genomes of the isolates were sequenced and sequence data submitted to the NCBI/EBI. Full details about this dataset can be found at https://doi.org/10.5285/299f89cd-7bbe-4428-9552-c51981c5f883
Hewitt, S.
University of Nottingham
Sarah.Hewitt@nottingham.ac.uk
author
Warry, A.
University of Nottingham
Andrew.Warry@nottingham.ac.uk
author
Dyer, P.S.
University of Nottingham
Paul.Dyer@nottingham.ac.uk
author
Avery, S.V.
University of Nottingham
Simon.Avery@nottingham.ac.uk
author
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
custodian
NERC Environmental Information Data Centre
info@eidc.ac.uk
publisher
Simon Avery
University of Nottingham
Simon.Avery@nottingham.ac.uk
pointOfContact
Soil
theme
GEMET - INSPIRE themes, version 1.0
2008-06-01
publication
Wild Yeast
Environmental stress resistance
Heterogeneity
otherRestrictions
no limitations
otherRestrictions
© Natural Environment Research Council
otherRestrictions
This resource is available under the terms of the Open Government Licence
otherRestrictions
If you reuse this data, you should cite: Hewitt, S., Warry, A., Dyer, P.S., Avery, S.V. (2019). Heterogeneity and environmental stress resistance of wild yeast isolates. NERC Environmental Information Data Centre https://doi.org/10.5285/299f89cd-7bbe-4428-9552-c51981c5f883
textTable
10
English
utf8
biota
2014-07-01
2014-07-31
-1.589
-1.574
54.972
54.983
Comma-separated values (CSV)
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
distributor
https://data-package.ceh.ac.uk/data/299f89cd-7bbe-4428-9552-c51981c5f883
Download the data
Download a copy of this data
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https://data-package.ceh.ac.uk/sd/299f89cd-7bbe-4428-9552-c51981c5f883.zip
Supporting information
Supporting information available to assist in re-use of this dataset
information
dataset
dataset
Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
2010-12-08
Soil-core samples were collected during July 2014 near the disused St. Anthony’s metal smelting works, at either contaminated (16.03 g kg-1 Pb, 10.87 mg Kg-1 Cd) or nearby less-contaminated (4.38 g kg-1 Pb, 1.49 mg Kg-1 Cd) areas. Coordinates and a description of the site are available at http://www.twsitelines.info/SMR/4192. Soil-core samples were diluted in the laboratory and spread to agar media for yeast isolation. Single colonies were subcultured and yeasts identified with ITS sequencing and RAPD PCR. 56 clonal isolates of Saitozyma podzolica were assayed for lead (Pb) and cadmium (Cd) resistance (IC50) and heteroresistance. Some isolates were also tested with zinc (Zn) Genomic DNA preparations submitted to NBAF-Liverpool for whole-genome sequencing. The sequence data were deposited with EBI/NCBI.