Identification

Title

Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform

Abstract

The data consists of the abundance of antibiotic resistant genes (ARG) in fresh cattle faeces from faecal samples taken from a cohort of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected in November 2016. The cohort of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Samples were collected from cattle as they entered housing for the winter, i.e. at weaning. Full details about this dataset can be found at https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/efcef5e3-09f2-4a23-ad19-53295a3171ce

name: Download the data

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/efcef5e3-09f2-4a23-ad19-53295a3171ce.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/efcef5e3-09f2-4a23-ad19-53295a3171ce

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

environment

Keywords

Keyword set

keyword value

Environmental Monitoring Facilities

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

North Wyke Farm Platform

Antibiotic resistant gene abundance

Geographic location

West bounding longitude

-3.93

East bounding longitude

-3.88

North bounding latitude

50.77

South bounding latitude

50.74

Temporal reference

Temporal extent

Begin position

2016-11-01

End position

2016-11-30

Dataset reference date

date type

publication

effective date

2020-05-18

Frequency of update

Quality and validity

Lineage

Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory faecal samples were pooled, such that for each farmlet (10 cattle) there were 3 samples, (3, 3, 4 cattle). This resulted in 9 faecal samples from which DNA was extracted using a commercially available extraction kit (MO BIO PowerSoil® DNA Isolation kit (catalogue No. 12888-100). The following adaption to the Power Soil protocol; at sample preparation samples were heated to 65 °C for 10 minutes prior to vortex. DNA was sequenced by the sequencing unit at the University of Exeter. The resulting metagenomic data was analysed at the Environmental Biotechnology Laboratory at the University of Hong Kong. The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677. Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

Comma-separated values (CSV)

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

If you reuse this data, you should cite: Hodgson, C.J. (2020). Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce

owner

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2022-05-18T12:21:29

Metadata language

eng