efcef5e3-09f2-4a23-ad19-53295a3171ce
English
ISO/IEC 8859-1 (also known as Latin 1)
dataset
dataset
Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
LA1 4AP
UK
info@eidc.ac.uk
pointOfContact
2022-05-18T12:21:29
UK GEMINI
2.3
OSGB 1936 / British National Grid
Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform
2020-05-18
publication
https://catalogue.ceh.ac.uk/id/efcef5e3-09f2-4a23-ad19-53295a3171ce
10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce
doi:
Hodgson, C.J. (2020). Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre 10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce
The data consists of the abundance of antibiotic resistant genes (ARG) in fresh cattle faeces from faecal samples taken from a cohort of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected in November 2016. The cohort of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Samples were collected from cattle as they entered housing for the winter, i.e. at weaning. Full details about this dataset can be found at https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce
Hodgson, C.J.
Rothamsted Research North Wyke
chris.hodgson@rothamsted.ac.uk
author
Dr. Chris Hodgson
Rothamsted Research North Wyke
chris.hodgson@rothamsted.ac.uk
pointOfContact
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
custodian
NERC Environmental Information Data Centre
info@eidc.ac.uk
publisher
Rothamsted Research
comms@rothamsted.ac.uk
owner
Environmental Monitoring Facilities
theme
GEMET - INSPIRE themes, version 1.0
2008-06-01
publication
Environmental survey
North Wyke Farm Platform
Antibiotic resistant gene abundance
otherRestrictions
no limitations
otherRestrictions
© Natural Environment Research Council
otherRestrictions
This resource is available under the terms of the Open Government Licence
otherRestrictions
If you reuse this data, you should cite: Hodgson, C.J. (2020). Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce
textTable
5
English
utf8
biota
environment
2016-11-01
2016-11-30
-3.93
-3.88
50.74
50.77
Comma-separated values (CSV)
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
distributor
https://data-package.ceh.ac.uk/data/efcef5e3-09f2-4a23-ad19-53295a3171ce
Download the data
Download a copy of this data
download
https://data-package.ceh.ac.uk/sd/efcef5e3-09f2-4a23-ad19-53295a3171ce.zip
Supporting information
Supporting information available to assist in re-use of this dataset
information
dataset
dataset
Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
2010-12-08
Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory faecal samples were pooled, such that for each farmlet (10 cattle) there were 3 samples, (3, 3, 4 cattle). This resulted in 9 faecal samples from which DNA was extracted using a commercially available extraction kit (MO BIO PowerSoil® DNA Isolation kit (catalogue No. 12888-100). The following adaption to the Power Soil protocol; at sample preparation samples were heated to 65 °C for 10 minutes prior to vortex. DNA was sequenced by the sequencing unit at the University of Exeter. The resulting metagenomic data was analysed at the Environmental Biotechnology Laboratory at the University of Hong Kong.
The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677.
Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.