Identification

Title

Oxidative stress markers in banded mongooses in Queen Elizabeth National Park, Uganda, 2017-2021

Abstract

The dataset includes markers of oxidative state in banded mongooses at Queen Elizabeth National Park, Uganda, in the period 2017-2021. Data are: red blood cell concentrations of glutathione; blood plasma concentrations of malondialdehyde; blood plasma concentrations of protein carbonyls; red blood cell activity of total superoxide dismutase; and whole milk concentrations of malondialdehyde. Full details about this dataset can be found at https://doi.org/10.5285/481c424e-f277-4eb7-9ee0-cbdb5532ef79

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/481c424e-f277-4eb7-9ee0-cbdb5532ef79

name: Download the data

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/481c424e-f277-4eb7-9ee0-cbdb5532ef79.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/481c424e-f277-4eb7-9ee0-cbdb5532ef79

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

Keywords

Keyword set

keyword value

Habitats and Biotopes

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

Antioxidants

Lipid peroxidation

Mungos mungo

Oxidative damage

Geographic location

West bounding longitude

29.669

East bounding longitude

30.361

North bounding latitude

0.194

South bounding latitude

-0.608

Temporal reference

Temporal extent

Begin position

2017-01-01

End position

2021-12-31

Dataset reference date

date type

publication

effective date

2022-03-08

Frequency of update

Quality and validity

Lineage

Individuals were blood sampled from the jugular vein under general anaesthesia. Samples were collected into EDTA-coated Vacutainers before plasma and erythrocytes were separated by centrifugation then snap frozen in liquid nitrogen within 10 minutes of collection. Samples were shipped to the UK within 3 months under cryogenic conditions, and stored at -80C until analysis. Analyses were performed blindly with respect to sample identity, and all steps were conducted on ice. All chemicals were HPLC grade, and chemical solutions were prepared using Milli-Q water. Assays were conducted within one year of collection. Malondialdehyde was determined by HPLC with fluorescence detection (Agilent 1000). Protein carbonyls, glutathione and superoxide dismutase were determined using colorimetric assay kits (Cayman Chemical Company) and a Molecular Devices plate reader (Spectramax M2).

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

pointOfContact

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2022-06-10T11:01:10

Metadata language

eng