QICS Data: Gene expression data of scallops Pecten maximus and mussels Mytilus edulis held in the vicinity of a sub-seabed CO2 release (2012)
The QICS project (Quantifying and Monitoring Potential Ecosystem Impacts of Geological Carbons Storage) was established to improve our understanding of the potential impacts of CO2 release on the environment and to develop tools and best practice for monitoring sub-seabed CCS reservoirs. To monitor the potential impact of a CO2 leak to surficial benthic megafauna, cages of bivalves (the common mussel Mytilus edulis Linnaeus, 1758 and the king scallop Pecten maximus (Linnaeus, 1758)) were deployed at the gas release site and at a reference site in the QICS experiment - both within Ardmucknish Bay, Oban, Scotland. Replicate individuals were sampled at six time points over a 125-day period, which spanned both the 37-day injection and recovery phases of the experiment, in order to establish impacts to molecular physiology. Samples of bivalves were also simultaneously sampled from a reference site within the bay in order to contrast changes in physiology induced by the gas release with naturally variability in the physiological performance of both species. There was no evidence of gene regulation of either selected carbonic anhydrases (CAx genes) or the alpha subunit of sodium potassium ATPAses (ATP1A genes) in individual bivalves collected from the CO2 gas release site, in either species. In the common mussel Mytilus edulis there was only evidence for changes with time in the expression of genes coding for different classes of carbonic anhydrase. It was concluded that the effects of the plume of elevated pCO2 on ion-regulatory gene transcription were negligible in both species. Pratt et al. 2015. No evidence for impacts to the molecular ecophysiology of ion or CO2 regulation in tissues of selected surface-dwelling bivalves in the vicinity of a sub-seabed CO2 release. International Journal of Greenhouse Gas Control. DOI:10.1016/j.ijggc.2014.10.001. QICS project website: www.bgs.ac.uk/qics/home.html.
dataset
http://www.bgs.ac.uk/ukccs/accessions/index.html#item8561
function: download
http://data.bgs.ac.uk/id/dataHolding/13606457
eng
Pratt et al. 2015. No evidence for impacts to the molecular ecophysiology of ion or CO2 regulation in tissues of selected surface-dwelling bivalves in the vicinity of a sub-seabed CO2 release. International Journal of Greenhouse Gas Control. DOI:10.1016/j.ijggc.2014.10.001. QICS project website: www.bgs.ac.uk/qics/home.html.
geoscientificInformation
publication
2008-06-01
Monitoring
Environmental impact
Carbon
Storage
revision
2011
NERC_DDC
-5.4200
-5.4200
56.4900
56.4900
revision
2002
Ardmucknish Bay [id=1214829]
2012-05-08
2012-09-09
creation
2012
notApplicable
Peer-reviewed paper to be published in the International Journal of Greenhouse Gas Control (in press). Will include original gene expression values and details of processing (citation to follow). Organism sampling, including tissue dissections conducted by Chris Hauton and Elizabeth Morgan; Ocean and Earth Science, University of Southampton Molecular methods conducted by Nicola Pratt; Ocean and Earth Science, University of Southampton This spreadsheet compiled by Hauton and Pratt, Ocean and Earth Science, University of Southampton. RNA extraction using TRIreagent (SigmaAldrich, Dorset, UK) according to manufacturer's instructions (Product No. T9424) RNA pellets air dried and diluted in DEPC-treated water (DEPC = diethyl pyrocarbonate) RNA purity assessed by measuring absorbance at 230nm, 260nm and 280nm using a NanoDrop spectrophotometer RNA quantity assessed using Bio-Rad (Herts, UK) Experion electrophoresis system according to manufacturer's instructions (Product No. 700-7104) RNA integrity (RQI) assessed using Bio-Rad Experion electrophoresis system according to manufacturer's instructions RNA DNase-treated using Promega (Hants, UK) RQ1 RNase-Free DNase, according to manufacturer's instructions (Product No. M6101) cDNA produced using LifeTechnologies (Strathclyde, UK) Superscript III Reverse Transcriptase according to manufacturer's instructions with oligo-dT priming (Product No. 18080044) qPCR carried out using a Qiagen (West Sussex) Rotorgene 3000 instrument qPCR Mastermix - Precision SY Mastermix with SYBR green (PrimerDesign, Hants, UK) (Product No. Precision-SY). qPCR conditions: 95 degrees for 10 minutes; 40 cycles of 95 degrees for 10 seconds, 60 degrees for 60 seconds qPCR amplicon specificity verified by melt curve analysis qPCR run files available upon request to Chris Hauton (ch10@noc.soton.ac.uk) Acknowledgements: field sampling and molecular lab work funded under the UK NERC QICS Project (NE/H013881/1)
publication
2011
false
See the referenced specification
publication
2010-12-08
false
See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF
.xls
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