a15c35d7-0247-4ea3-820b-f6f20cc5ffed
English
ISO/IEC 8859-1 (also known as Latin 1)
dataset
dataset
Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
LA1 4AP
UK
info@eidc.ac.uk
pointOfContact
2022-05-20T10:41:58
UK GEMINI
2.3
WGS 84
Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high glucose concentration added as a carbon source in the Conwy catchment, North Wales, UK (2016)
2018-03-19
publication
2016-11-30
creation
https://catalogue.ceh.ac.uk/id/a15c35d7-0247-4ea3-820b-f6f20cc5ffed
10.5285/a15c35d7-0247-4ea3-820b-f6f20cc5ffed
doi:
Glanville, H.C., De Sosa, L.L. , Marshall, M.R., Cooper, D.M., Jones, D.L. (2018). Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high glucose concentration added as a carbon source in the Conwy catchment, North Wales, UK (2016). NERC Environmental Information Data Centre 10.5285/a15c35d7-0247-4ea3-820b-f6f20cc5ffed
Time series data of carbon release in disintegrations per minute are presented for different nitrogen and phosphorus treatments with a high glucose concentration substrate added as a carbon source to soil samples from six depths (0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres). Soil cores were collected from a field experiment in the Conwy catchment in July 2016 and returned the laboratories of the School of Environment, Natural Resources and Geography, Bangor University. A high molecular weight substrate was added as a carbon source to the samples and the rate of 14C-substrate mineralization measured.
All the work was carried out by trained members of staff from Bangor University and the Centre for Ecology & Hydrology.
The measurements were taken to improve understanding of the relationship between microbial activity and soil properties and depth of sampling, under differing nutrient availability.
The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf.
Full details about this dataset can be found at https://doi.org/10.5285/a15c35d7-0247-4ea3-820b-f6f20cc5ffed
Dr. Helen Glanville
Bangor University
h.c.glanville@keele.ac.uk
pointOfContact
Glanville, H.C.
Bangor University
h.c.glanville@keele.ac.uk
author
De Sosa, L.L.
Bangor University
afs411@bangor.ac.uk
author
Marshall, M.R.
Centre for Ecology & Hydrology
m.marshall@bangor.ac.uk
author
Cooper, D.M.
Centre for Ecology & Hydrology
enquiries@ceh.ac.uk
author
Jones, D.L.
Bangor University
d.jones@bangor.ac.uk
author
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
custodian
NERC Environmental Information Data Centre
info@eidc.ac.uk
publisher
notPlanned
Environmental Monitoring Facilities
theme
GEMET - INSPIRE themes, version 1.0
2008-06-01
publication
Environmental survey
Turf2Surf (T2S)
Macronutrient Cycling Programme (MCP)
liquid scintillation counter
Soil core
Conwy Catchment
North Wales
otherRestrictions
no limitations
otherRestrictions
This resource is made available under the terms of the Open Government Licence
otherRestrictions
© Bangor University
otherRestrictions
© UK Centre for Ecology & Hydrology
otherRestrictions
If you reuse this data, you should cite: Glanville, H.C., De Sosa, L.L. , Marshall, M.R., Cooper, D.M., Jones, D.L. (2018). Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high glucose concentration added as a carbon source in the Conwy catchment, North Wales, UK (2016). NERC Environmental Information Data Centre https://doi.org/10.5285/a15c35d7-0247-4ea3-820b-f6f20cc5ffed
textTable
10
English
utf8
environment
2016-07-15
2016-09-30
-3.784
-3.781
53.201
53.204
Comma-separated values (CSV)
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
distributor
https://data-package.ceh.ac.uk/data/a15c35d7-0247-4ea3-820b-f6f20cc5ffed
Download the data
Download a copy of this data
download
https://data-package.ceh.ac.uk/sd/a15c35d7-0247-4ea3-820b-f6f20cc5ffed.zip
Supporting information
Supporting information available to assist in re-use of this dataset
information
dataset
dataset
Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
2010-12-08
Soil cores were collected from the field site and returned to laboratories of the School of Environment, Natural Resources and Geography, Bangor University.
In the laboratory soil cores were divided into depth intervals of 0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres and passed through a 5 millimetre sieve in order to remove stones and any plant material and to ensure sample homogeneity.
To measure the rate of 14C-substrate mineralization, 5 grams of soil (dry weight equivalent to account for soil water content variability down the soil profile) was placed into sterile 50 ml polypropylene tubes. To determine the rate of 14CO2 evolution, 50 microlitres (µl) of 14C-glucose labelled nutrient solution was added to the soil surface. Immediately after nutrient addition, a vial containing 1 millilitre (ml) Sodium Hydroxide (NaOH) trap (1 Molar (M)) was added into the polypropylene tubes to capture 14CO2 evolved. The tubes were hermetically sealed and incubated at 10 degrees Celsius to represent the mean annual temperature of the catchment. The NaOH traps were changed after 0.5, 1, 2, 4, 6, 24, 48, 72, 96, 120, 144, 168, 192, 336, 504, 672, 840 and 1008 hours and then weekly up to six weeks after initial 14C-labelling for the glucose-C additions. On removal, the NaOH traps were mixed with Optiphase HiSafe 3® liquid scintillation fluid (PerkinElmer Inc.) and the amount of 14CO2 captured was determined using a Wallac 1404 liquid scintillation counter (Wallac EG & G).
Data were entered into an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC