Identification

Title

Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps v2

Abstract

Data consists of gene expression estimates and encapsulation rates in wild caught Drosophila melanogaster larvae following exposure to different treatments. Treatments include injection with wasp homogenate, injection with oil and no injection. Also provided are functional enrichment categories for differentially expressed genes and library generation and read mapping metrics. The data were produced under the grant: NE/P00184X/1 Why does Drosophila vary in susceptibility to parasitoid wasps. Full details about this dataset can be found at https://doi.org/10.5285/06ea87f3-476d-40fd-acce-e6923e786d48

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/06ea87f3-476d-40fd-acce-e6923e786d48

name: Download the data

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/06ea87f3-476d-40fd-acce-e6923e786d48.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/06ea87f3-476d-40fd-acce-e6923e786d48

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

Keywords

Keyword set

keyword value

Habitats and Biotopes

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

originating controlled vocabulary

title

Wikidata

reference date

date type

creation

effective date

2012-10-29

Keyword set

keyword value

RNA-sequencing

parasitoid wasp

differential gene expression

fat body

encapsulation

hemocyte

Evolutionary genetics

insect genomics

Geographic location

West bounding longitude

-1.464

East bounding longitude

1.768

North bounding latitude

52.974

South bounding latitude

51.232

Temporal reference

Temporal extent

Begin position

2017-10-01

End position

2017-10-31

Dataset reference date

date type

publication

effective date

2022-03-08

Frequency of update

Quality and validity

Lineage

Drosophila melanogaster larvae obtained from a wild caught population were exposed injected with wasp homogenate, oil or no manipulation was made. Encapsulation rates were measured in each case in the fly larvae. For RNA-sequencing, hemocyte and fat body tissues were isolated from third instar larvae 24 hours post experimental manipulation. RNA was isolated for sequencing. Sequenced reads were trimmed, mapped to the dm6 genome, number of reads mapping to each gene was counted, differential expression tests were applied, and functional enrichment was done.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

owner

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2022-05-18T12:21:12

Metadata language

eng