Identification

Title

Global river cellulose decomposition assay data 2016-17

Abstract

This dataset contains information regarding mountain river sites sampled in 2016-17. It includes: 1) physicochemical parameters of the river sites sampled, 2) tensile-strength loss data of a standardised cotton strip assay as a proxy for the decomposition of cellulose in mountain rivers, 3) molecular next generation sequencing data of the fungal Internal Transcribed Spacer region (ITS) and Cellobiohydrolase I gene (cbhI) sampled from the cotton strip decomposition assay. Field observations were made in Alaska, Austria, Ecuador, France, New Zealand and Norway to investigate how decreasing catchment glacier cover influences the fungal decomposition of organic matter in mountain rivers. Full details about this dataset can be found at https://doi.org/10.5285/fec704d2-ee6a-427b-9345-850dd96ff1b4

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/fec704d2-ee6a-427b-9345-850dd96ff1b4

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/fec704d2-ee6a-427b-9345-850dd96ff1b4.zip

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/fec704d2-ee6a-427b-9345-850dd96ff1b4

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

inlandWaters

Keywords

Keyword set

keyword value

Environmental Monitoring Facilities

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Geographic location

West bounding longitude

-180

East bounding longitude

180

North bounding latitude

South bounding latitude

-90

Temporal reference

Temporal extent

Begin position

2016-01-01

End position

2017-12-31

Dataset reference date

date type

publication

effective date

2021-01-27

Frequency of update

Quality and validity

Lineage

In the field, physicochemical parameters were obtained using a combination of in-situ probe measurements and geomorphological assessment, alongside collection of river water for ex-situ determination of some variables. A standardised cellulose (cotton strip) decomposition assay was deployed at each river site. In the laboratory, a tensiometer (University of Leeds) was used to determine the tensile-strength loss for incubated and control cotton strips, as a proxy for the decomposition of cellulose. Tensile-strength loss values were standardised by days of incubation and water temperature. DNA was extracted from subsamples of the cotton strips and next generation sequencing (qPCR, PCR) (University of Essex) performed to estimate the abundance of fungi (ITS), subgroups of fungi (saprotrophs, Ascomycota, Tetracladium) and a fungal gene (cbhI). Molecular data was subjected to quality control prior to taxonomic classification and assignment of trophic mode.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

organisation name

email address

sarahcfell88@gmail.com

web address

https://orcid.org/0000-0001-5753-276X

description: ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.

function: information