Identification

Title

Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD)

Abstract

[This dataset is embargoed until October 28, 2025]. This dataset contains information about the development of a paper analytical device for the detection of the microcystin toxin. Water samples were collected weekly from Rostherne Mere and Tatton Park Lake, Cheshire between 20/07/2022 and 12/10/2022. Samples were analysed for the presence of microcystin-producing genes and released microcystin. A paper analytical device incorporating a protein phosphatase inhibition assay was also used to monitor microcystin levels. Preliminary user evaluation of the paper analytical devices and associated mobile photo applications is also provided. The work was supported by the Natural Environment Research Council (Grant NE/X011607/1). Full details about this dataset can be found at https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491

Resource type

dataset

Resource locator

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/5fe25b28-f10b-467b-8812-0b1ee6fd7491

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

environment

Keywords

Keyword set

keyword value

Environmental Monitoring Facilities

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Geographic location

West bounding longitude

-2.403

East bounding longitude

-2.35

North bounding latitude

53.361

South bounding latitude

53.307

Temporal reference

Temporal extent

Begin position

2022-06-20

End position

2022-10-12

Dataset reference date

date type

publication

effective date

2024-06-12

Frequency of update

Quality and validity

Lineage

Water samples were taken on a weekly basis from three sampling locations at each site. Samples were filtered through a 0.45 µm filter and DNA was extracted from the filter paper using a Qiagen PowerWater kit before being analysed using real-time PCR for detection of the mycE gene, alongside no template controls. Water samples were analysed using an ENZO Life Sciences Microcystins (Adda specific) ELISA kit, alongside microcystin reference standards and controls. For the development of the paper analytical device, protein phosphatase 1a was produced using protein expression and confirmed using spectrophotometry. Efficiency was evaluated by comparison to commercially available products and comparing absorbance values for the conversion of pNPP. The paper analytical device incorporating the protein phosphatase inhibition assay was then used to detect microcystin. Colourimetric change was recorded for samples and controls using Image J. Preliminary user evaluation of the paper analytical device was obtained using qualitative feedback.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

Comma-separated values (CSV)

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Embargoed

Constraint set

Limitations on public access

Constraint set

Limitations on public access

If you reuse this data, you should cite: Hill, M., Jenkinson, L.A., Dean, A.P., Redfern, J., Shaw, K.J. (2024). Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD). NERC EDS Environmental Information Data Centre https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491

Responsible organisations

Responsible party

organisation name

Manchester Metropolitan University

email address

m.hill@mmu.ac.uk

responsible party role

author

Responsible party

organisation name

Manchester Metropolitan University

email address

lauren.a.jenkinson@stu.mmu.ac.uk

responsible party role

author

Responsible party

organisation name

Manchester Metropolitan University

email address

andrew.dean@mmu.ac.uk

web address

https://orcid.org/0000-0001-6893-5118

description: ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.

function: information

responsible party role

author

Responsible party

organisation name

Manchester Metropolitan University

email address

j.redfern@mmu.ac.uk

web address

https://orcid.org/0000-0003-0958-683X

function: information

responsible party role

author

Responsible party

organisation name

Manchester Metropolitan University

email address

k.shaw@mmu.ac.uk

web address

https://orcid.org/0000-0001-9241-4195

function: information

responsible party role

author

Responsible party

organisation name

Manchester Metropolitan University

email address

k.shaw@mmu.ac.uk

responsible party role

pointOfContact

Responsible party

organisation name

email address

info@eidc.ac.uk

responsible party role

publisher

Responsible party

organisation name

email address

info@eidc.ac.uk

responsible party role

custodian

Responsible party

organisation name

email address

EMAIL NOT PROVIDED

responsible party role

owner

Metadata on metadata

Metadata point of contact

organisation name

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

web address

https://eidc.ac.uk/

description: The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences.

function: information

responsible party role

pointOfContact

Metadata date

2025-03-21T13:22:04

Metadata language

eng