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HT-qPCR data on microbial DNA markers in archived bathing water samples

Publisher
Environment Agency
Updated
7 October 2026
Topic
Not set

Summary

This dataset relates to the Environment Agency Chief Scientist report ‘Evaluating microbial DNA markers in archived bathing water samples’ (SC240046) which describes in detail the data collection, processing and analysis for this dataset.

The dataset relates to 235 bathing water samples originally collected in 2023 as part of the statutory monitoring programme which were stored as frozen filter membranes. DNA was extracted from these archived samples and analysed using high throughput quantitative polymerase chain reaction (HT-qPCR) for 36 microbial gene markers. These microbial markers were specific for a targeted set of pathogens, microbial source tracking, bacteriophages and other taxonomic species.

Data includes:

  • Sample timestamps and measurement details of E. coli, intestinal Enterococci and water temperature from statutory samples.
  • Data (raw Ct counts and gene marker abundances) from the samples analysis by the HT-qPCR instrument.

See the following files for information on attributes and their plain English descriptions: • Metadata_for_collated_result_data.csv • Metadata for raw HT-qPCR data.txt Attribution statement: © Environment Agency copyright and/or database right 2026. All rights reserved.

Data links

Link Format Preview Updated
Relative abundance.csv CSV Preview 7/10/2026
Evaluating microbial DNA markers in archived bathing water samples – 7/10/2026
Metadata_for_collated_result_data.csv CSV Preview 7/10/2026
Metadata for raw HT-qPCR data.txt TXT – 7/10/2026
Copies in 10 uL DNA.csv CSV Preview 7/10/2026
Collated_result_data.csv CSV Preview 7/10/2026
136147A.txt TXT – 7/10/2026
136148A.txt TXT – 7/10/2026
136142A.txt TXT – 7/10/2026
136127A.txt TXT – 7/10/2026
136104A.txt TXT – 7/10/2026

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Additional information

Source Metadata
XML, HTML
Date added
29 September 2026
Harvest GUID
47cb1ece-0225-466c-ab3a-a389f15edcbb
Extent (Latitude)
55.816° to 49.943°
Extent (Longitude)
-6.236° to 2.072°
Spatial reference system
http://www.opengis.net/def/crs/EPSG/0/27700
Dataset reference date (creation)
2026-07-17
Dataset reference date (publication)
2026-09-29
Dataset reference date (revision)
2026-09-02
Frequency of update
notPlanned
Access constraints
There are no public access constraints to this data. Use of this data is subject to the licence identified.
Responsible party
Environment Agency (pointOfContact)
ISO 19139 resource type
dataset
Metadata language
eng

Contact

Enquiries

DSPcustomerforum@environment-agency.gov.uk

Freedom of information (FOI) requests

enquiries@environment-agency.gov.uk

Freedom of Information (FOI) requests

Licence information

Open Government Licence