These datasets contain aphid/parasitoid abundances and glucosinolate concentrations associated with Brassica napus plants within eight field-based diesel exhaust and ozone fumigation rings located at the University of Reading’s Sonning farm. Full details about this dataset can be found at https://doi.org/10.5285/b28c13f2-7355-4c4b-8c34-10470e1772d1