Data comprise concentrations of elements in ashed fish sampled from lakes in the English Lake District in 2012 and 2013. Fish were collected from three lakes (Windermere, Bassenthwaite Lake and Derwent Water) by the Centre for Ecology & Hydrology (CEH) Lake Ecosystems group. Fish species collected were Roach (Rutilus rutilus), Perch (Perca fluviatilis), Ruffe (Gymnocephalus cernuus), Brown trout (Salmo trutta), Pike (Esox lucius) and Vendace (Coregonus albula). All samples were ashed prior to analysis by ICPMS or ICPOES to determine elemental concentrations. Full details about this dataset can be found at https://doi.org/10.5285/ed90df1b-462c-46bb-afbd-59794fb03f6b
