The data contain measurements of the morphological shape of threespine stickleback from wild, F1 (filial generation 1) lab-reared, and F2-lab (filial generation 2) reared individuals. These last two groups were bred and reared from eggs in the lab. The files are in tps format and so within these contain the information regarding the population, rearing temperature, and scale factor for each specimen. The populations used are coded in a short form format but we also provide a key to decipher these names in csv format. Photos are also made available with corresponding tps files for F2 hybrid fish. Full details about this dataset can be found at https://doi.org/10.5285/a566fc20-a371-42a2-8530-9a3cade09261
