The dataset contains time series of dissolved oxygen dynamics and near-streambed light availability from selected riverine sites within the Hampshire Avon catchment (UK). Six rivers within sub-catchments of contrasting geology (clay, greensand, chalk) and associated river morphology were investigated. Data were obtained from field-based measurements in seasonal campaigns conducted between spring 2013 and winter 2014. Full details about this dataset can be found at https://doi.org/10.5285/c1f14c54-ffe1-4581-b479-897ff9262e98