Prevalence of quinolone qnrS resistance gene in the aquatic environment from the Avon river catchment area receiving treated wastewater from 5 wastewater treatment plants (WWTPs), serving 1.5 million people and accounting for 75% of inhabitants living in the catchment area in the South West of England. Funded by NERC Grant NE/N019261/1 Full details about this dataset can be found at https://doi.org/10.5285/102f8141-2a9a-4ffd-89f6-961af36ddcb3