This data set includes soil chemical, physical and microbial properties collected across a two-century glacial chronosequence across six streams in Glacier Bay, Southeast Alaska, U.S.A. We measured soil potential nitrification, denitrification, as well as stable isotopes (delta-15N and delta-13C) of leaves and soil to establish how physical and biological changes associated with ecosystem development interact to determine rates of carbon (C) and nitrogen (N) turnover. Secondly, how these interactions were reflected in the isotopic signature of vegetation and SOM. Data were collected between June 2011 and September 2013 during summer sampling campaigns. Full details about this dataset can be found at https://doi.org/10.5285/7c4c1ed0-3d45-4b7f-8007-b81f6028d686
