The dataset contains carbon dioxide and methane emissions, as well as resorufin production (as a proxy for microbial metabolic activity) and dissolved oxygen concentrations, resulting from laboratory incubation experiments of streambed sediments. The sediments were collected from the upper 10 centimetres of the streambed in the River Tern and the River Lambourn in September 2015, with three samples collected from each river. These samples were collected from three areas: silt-dominated sediment underneath vegetation (fine), sand-dominated sediment from unvegetated zones (medium) and gravel-dominated sediment from unvegetated zones (coarse). The sediment was used in laboratory incubation experiments to determine the effect of temperature, organic matter content, substrate type and geological origin on streambed microbial metabolic activity, and carbon dioxide and methane production. The work was carried out as part of a Natural Environment Research Council (NERC) funded PhD (NERC award number 1602135). The work was also part funded through the Seventh Framework Programme (EU grant number 607150). Full details about this dataset can be found at https://doi.org/10.5285/3a0a5132-797c-4ed5-98b9-1c17eaa2f2b7
