Dataset contains images recorded during a trial of Automated Monitoring of Insects (AMI), Automoth and Diopsis camera systems. The images were taken at five sites in Barro Colorado Island, Panama, during night-time hours from the 23rd to the 26th of January 2023. The parties involved in data collection are listed in the author section. Full details about this dataset can be found at https://doi.org/10.5285/b088d4bd-4abb-46d4-bc90-f4e58c65f324