Data from two small streams, two rivers and rainfall fractions in the Western Amazonian basin at Tambopata National Reserve in Madre de Dios region, Peru. Data presented are nutrients (calcium, magnesium, potassium, sodium, total soluble phosphorus and silica) and fluvial carbon - dissolved inorganic carbon (DIC) and its isotopic composition δ13C-DIC, dissolved organic carbon (DOC) and particulate organic carbon (POC). Samples were collected during the period from February 2011 to May 2012 targeting both wet and dry seasons. Samples for DIC samples were collected using pre-acidified evacuated Exetainers. Established standard methods were used to take samples for DOC and nutrients. Established standard methods were used to analyse samples for DIC, DOC and nutrients These methods are outlined in the lineage. The samples were taken to understand the hydrological controls on the carbon concentrations and fluxes during different flow conditions. The data collection was carried out as part of the Natural Environment Research Council funded Amazonica project. Full details about this dataset can be found at https://doi.org/10.5285/ee1b9eb7-6fbd-4dd5-8f8f-e07d32c057e4
