The dataset contains soil chemistry data from the Wakehurst Ecosystem Observatory and associated projects, maintained by the Royal Botanic Gardens (RBG), Kew. The variables measured include carbon, nitrogen, pH, bulk density, dry matter, organic matter, sodium, calcium, magnesium, potassium, sulphate, phosphorus, conductivity, cation exchange capacity, and soil texture. Each row records a single soil sample from a specific location at a defined depth band, with associated chemical measurements and metadata. The dataset contains 907 soil samples collected between November 2021 and November 2024 across 10 distinct project campaigns. The data were primarily collected to establish baseline soil conditions across the Wakehurst landscape, supporting research into soil carbon dynamics, fungal communities, and ecosystem function. Full details about this dataset can be found at https://doi.org/10.5285/a6594b8e-3f42-432a-bfb3-7bd7a15ac03a
