The dataset contains 323 diverse wheat accessions under two N regimes (N100 and N50) across two environments over two consecutive years, 2021-2023. All 323 genotypes were planted in two different Locations: 1) University of Agriculture, Faisalabad (31.4396° N, 73.0768° E), and 2) National Institute of Genomics and Advanced Biotechnology (NIGAB), Islamabad (33.6736° N, 73.1240° E). The experiments were laid down for two years; Yr1) Rabi-2021-22 and Yr2) Rabi-2022-23. Alpha lattice was used as an experimental design with 4 replications at location 1 and 3 replications at location 2. Treatments were applied as 100% of the recommended N dose at a rate of 120 kg ha-1 (N100) and 50% of the recommended dose at a rate of 60 kg ha-1(N50). Urea was used as N source and applied in three splits as basal dose, at stem elongation stage and at the heading stage. Standard irrigation and crop management practices were followed for all environments. The following parameters were measured: days to flowering, flag leaf area (cm2), peduncle length (cm), spike length (cm), plant height (cm), straw yield (g), grain yield (g) and thousand grain weight (g). The work was supported by the Natural Environment Research Council (Grant NE/S009019/1). Full details about this dataset can be found at https://doi.org/10.5285/7aa32225-f277-4ff6-bc73-7fe6123748cc
