This dataset contains over 4000 faecally-contaminated environmental samples collected over 2 years across 53 dairy farms in England. The samples were analysed for E. coli resistance to amoxicillin, streptomycin, cefalexin, tetracycline and ciprofloxacin and detection of resistant strains is presented in the dataset as a binary result, along with mechanisms of resistance to third generation cephalosporins where relevant. In addition there is comprehensive farm management data including antibiotic usage data. Full details about this dataset can be found at https://doi.org/10.5285/c9bc537a-d1c5-43a0-b146-42c25d4e8160
