This dataset contains information about planktonic bacterial quantity at the drinking water pumping stations installed in three different aquifer types of England. The aim of data collection was to assess spatial and seasonal variation of groundwater bacterioplankton distribution and their dependence on organic matter, nutrient concentration and local hydrogeology. Untreated groundwater samples were collected from the intergranular sandstone aquifer of English Midlands and East Midlands, the dual porosity chalk aquifer of South East England and the karstic limestone aquifer of the Cotswolds. For temporal variation in each aquifer, the same aquifers were sampled during pre-recharge season in September-October 2022 and post-recharge season in January-February 2023. Full details about this dataset can be found at https://doi.org/10.5285/b27ce2ee-89ee-428d-bcd5-4abd97141d62
