This dataset contains hourly water temperature data and hourly air temperature data of an experimental mesocosm facility from 21st April to 7th November 2023. The sixteen mesocosms (1 m deep, 2 m diameter) were filled with water from Windermere. The water temperature was measured every five minutes and an hourly average was calculated. Air temperature was measured by a weather station within the mesocosm compound. The experiment aimed to investigate different N:P nutrient ratios and water temperature was measured as this is an important factor needed to understand the results. Full details about this dataset can be found at https://doi.org/10.5285/406aee8f-3b85-4be6-ae7d-c92c8168a06f