The dataset contains denitrification, anaerobic ammonium oxidation (anammox) and nitrification activity of riverine sediments in the Hampshire Avon catchment (UK). Six rivers within sub-catchments of contrasting geology (clay, sand, chalk) were investigated. Data were obtained via direct, field-based measurements in seasonal campaigns conducted between spring 2013 and winter 2014. Also included are chemical parameters determined in porewaters prior to in situ rate measurements. Full details about this dataset can be found at https://doi.org/10.5285/9d17d85d-eae2-4056-ad77-f1bc1c368b56