This dataset contains operational taxonomic units for soil bacteria collected from various land use categories in the Wolf and Tamar catchments in South west England. A range of soils were targeted from the Tamar region comprising a range of land uses. Approximate location of sampling sites was determined from maps to provide good spatial coverage of the catchment. Exact sites were determined in the field, considering accessibility and other logistic, and soils taken. Full details about this dataset can be found at https://doi.org/10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b
