Greenhouse gas concentrations and physio-chemical water properties for the Clyde estuary in Scotland to support understanding of the GHG sources and sinks and their associated mechanisms in a highly stratified, temperate urban estuary. These measurements look at the changes in GHG from a single point in the middle of the estuary (Breahead pontoon) through a tidal cycle at both the surface and bed so the impact of tidal river flow and stratification can be understood. Full details about this dataset can be found at https://doi.org/10.5285/7f5b221e-d251-4bac-9252-16b4553dc345