Data comprise site location, soil chemistry (pH, soil moisture), soil radionuclide activity concentrations (the isotopes measured were: Americium-241, Caesium-137, Plutonium-238, -239 and -240, Strontium-90 (K-40 and U-238 activity concentrations were estimated from stable element data) and soil biological activity (derived from application of bait lamina sticks) at 18 sites in the Chernobyl Exclusion Zone (CEZ), Ukraine in 2016; data for four sites in 2005 are also presented. Estimate absorbed radionuclide dose rates to soil invertebrates and bacteria are also presented. The primary purpose of these data was to enable an evaluation of the potential impact of radiation on soil organisms. The work was carried out by UKCEH, Chernobyl Centre for Nuclear Safety and the University of Salford. Funding for this work was via the TREE project funded by NERC, Environment Agency and Radioactive Waste Management Ltd. under the RATE programme. Full details about this dataset can be found at https://doi.org/10.5285/19babe1c-b3a3-488c-b4fe-ebb4ab9237d8
