Data comprise measurements of spectral reflectance for quaking aspen (Populus tremuloides Michx.) trees at a range of sites in southwestern Colorado near the town of Crested Butte. Spectra were measured in three different ways: hyperspectral measurements of leaves, hyperspectral measurements of bark, and multispectral measurements of canopies. The first two measurements were made using a handheld spectrometer, while the latter were made via airborne imaging from an unmanned aerial system. In addition to these reflectance data, the dataset also includes information on the genotype and/or ploidy level of each sample, as determined by either DNA microsatellite analysis or flow cytometry. All samples are georeferenced. The data include extracted data and raw imagery. Full details about this dataset can be found at https://doi.org/10.5285/d663aeb9-1e3e-40f7-ab9e-494e7646faeb
